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high capacity complementary deoxyribonucleic acid cdna reverse transcription rt kit  (Thermo Fisher)


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    Thermo Fisher high capacity complementary deoxyribonucleic acid cdna reverse transcription rt kit
    High Capacity Complementary Deoxyribonucleic Acid Cdna Reverse Transcription Rt Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/deoxyribonucleic+acid+cdna/Deoxyribonucleic+acid/pm42019360-59-0-12
    Average 99 stars, based on 1 article reviews
    high capacity complementary deoxyribonucleic acid cdna reverse transcription rt kit - by Bioz Stars, 2026-10
    99/100 stars

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    Reverse Transcription:

    Article Title: Microfluidic device for cerebrovascular simulation and high-efficiency blood-brain barrier simulation system comprising same
    Article Snippet: The primary antibodies used in the present disclosure are as follows: anti-CD31 (1:200, Millipore, Billerica, MA, USA), anti-Tuj1 (1:150, Cell Signaling Technology, Danvers, MA, USA), anti-GFAP (1:200, Millipore), anti-O4 (1:500, Millipore), anti-NG2 (1:100, Abcam, Cambridge, UK), anti-M-1 (1:100, Invitrogen) and anti-glucuronoxylomannan (anti-GXM, 1:500, 18B7 mouse monoclonal antibody donated by Dr. Arturo Casadevall in Johns Hopkins University). .. Quantitative Reverse Transcription Polymerase Chain Reaction (qPCR) To compare relative gene expression levels between groups, total RNAs were collected from each sample (n=3, technical replicates=3 per group) using an RNeasy mini kit (Qiagen, Chatsworth, CA, USA), and a complementary deoxyribonucleic acid (cDNA) was synthesized using a cDNA synthesis kit (Takara, Shiga, Japan) as directed by the manufacturer. qPCR analysis was performed on a StepOnePlus Real-Time PCR System (Applied Biosystems) using a TaqMan gene expression assay and a TaqMan Fast Universal PCR Master Mix (Applied Biosystems, Foster City, CA, USA). .. For gene expression analysis in human NSCs, a TaqMan gene expression assay with human-specific primers for the nestin (Hs00707120_s1), Tuj1 (Hs00801390_s1), GFAP (Hs00909238_g1), Olig2 (Hs00300164_s1) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH; Hs02758991_g1) were used.

    Article Title: Remimazolam-induced anesthesia does not affect circadian rhythms significantly in mice.
    Article Snippet: .. 409 Quantitative real-time polymerase chain reaction (q-PCR): Total RNA was 410 extracted from SCN samples using an RNeasy Mini Kit (Qiagen) and reverse-411 transcribed into the complementary deoxyribonucleic acid (cDNA) using a 412 QuantiTect Reverse Transcription Kit (Qiagen). q-PCR was performed using a 413 StepOnePlus Real-Time PCR system (Applied Biosystems, Foster, CA, USA) and a 414 QuantiTect SYBR Green PCR Kit (Qiagen). ..

    Article Title: miR-320a Regulates Placenta Endothelial Function After Fetal Cardiopulmonary Bypass via the ATG7-SIRT1/FOXO1 Pathway.
    Article Snippet: .. Total RNA was reverse-transcribed to complementary deoxyribonucleic acid (cDNA) according to the manufacturer’s protocol (EasyScript First-Strand cDNA Synthesis SuperMix, TransGen Biotech, China). qRT-PCR was performed on a qPCR machine (ABI PRISM® 7500 Sequence Detection System, Applied Biosystems, USA) with SYBR Green qPCR SuperMix (11733–046, Invitrogen, Thermo Scientific, USA). ..

    Article Title: Thrombospondin-1 (THBS1) is dysregulated in preeclampsia.
    Article Snippet: Once extracted, RNA was quantified with a Nanodrop ND 1000 spectrophotometer (NanoDrop Technologies Inc, Wilmington, DE, USA). .. mRNA extracted from cells and tissues was converted to complementary deoxyribonucleic acid (cDNA) using the Applied Biosystems high-capacity cDNA reverse transcriptase kit (Life Technologies, Carlsbad, USA) according to manufacturer’s instructions. .. Equal amounts of RNA were reverse transcribed into cDNA on the Applied Biosystems MiniAmp Thermal Cycler (ThermoFisher Scientific) following the manufacturer’s instructions.

    Article Title: miR-320a Regulates Placenta Endothelial Function After Fetal Cardiopulmonary Bypass via the ATG7-SIRT1/FOXO1 Pathway
    Article Snippet: .. Total RNA was reverse-transcribed to complementary deoxyribonucleic acid (cDNA) according to the manufacturer’s protocol (EasyScript First-Strand cDNA Synthesis SuperMix, TransGen Biotech, China). qRT-PCR was performed on a qPCR machine (ABI PRISM® 7500 Sequence Detection System, Applied Biosystems, USA) with SYBR Green qPCR SuperMix (11733–046, Invitrogen, Thermo Scientific, USA). ..

    Article Title: B7 homolog 6 promotes the killing activity of natural killer cells against cervical cancer through the downstream ERK pathway of NKp30
    Article Snippet: Total RNA was extracted from cultured cells using TRIzol ® reagent (cat. no. 213407; Invitrogen; Thermo Fisher Scientific, Inc.). .. Purified RNA was then reverse-transcribed to complementary deoxyribonucleic acid (cDNA) using RevertAid First Strand cDNA Synthesis Kit purchased from Thermo Fisher Scientific, Inc. Next, RT-qPCR was performed using FastStart Universal SYBR Green Master Mix (cat. no. 04913850001; Roche Diagnostics, Basel, Switzerland), following the manufacturer’s instructions. ..

    Polymerase Chain Reaction:

    Article Title: Microfluidic device for cerebrovascular simulation and high-efficiency blood-brain barrier simulation system comprising same
    Article Snippet: The primary antibodies used in the present disclosure are as follows: anti-CD31 (1:200, Millipore, Billerica, MA, USA), anti-Tuj1 (1:150, Cell Signaling Technology, Danvers, MA, USA), anti-GFAP (1:200, Millipore), anti-O4 (1:500, Millipore), anti-NG2 (1:100, Abcam, Cambridge, UK), anti-M-1 (1:100, Invitrogen) and anti-glucuronoxylomannan (anti-GXM, 1:500, 18B7 mouse monoclonal antibody donated by Dr. Arturo Casadevall in Johns Hopkins University). .. Quantitative Reverse Transcription Polymerase Chain Reaction (qPCR) To compare relative gene expression levels between groups, total RNAs were collected from each sample (n=3, technical replicates=3 per group) using an RNeasy mini kit (Qiagen, Chatsworth, CA, USA), and a complementary deoxyribonucleic acid (cDNA) was synthesized using a cDNA synthesis kit (Takara, Shiga, Japan) as directed by the manufacturer. qPCR analysis was performed on a StepOnePlus Real-Time PCR System (Applied Biosystems) using a TaqMan gene expression assay and a TaqMan Fast Universal PCR Master Mix (Applied Biosystems, Foster City, CA, USA). .. For gene expression analysis in human NSCs, a TaqMan gene expression assay with human-specific primers for the nestin (Hs00707120_s1), Tuj1 (Hs00801390_s1), GFAP (Hs00909238_g1), Olig2 (Hs00300164_s1) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH; Hs02758991_g1) were used.

    Article Title: Remimazolam-induced anesthesia does not affect circadian rhythms significantly in mice.
    Article Snippet: .. 409 Quantitative real-time polymerase chain reaction (q-PCR): Total RNA was 410 extracted from SCN samples using an RNeasy Mini Kit (Qiagen) and reverse-411 transcribed into the complementary deoxyribonucleic acid (cDNA) using a 412 QuantiTect Reverse Transcription Kit (Qiagen). q-PCR was performed using a 413 StepOnePlus Real-Time PCR system (Applied Biosystems, Foster, CA, USA) and a 414 QuantiTect SYBR Green PCR Kit (Qiagen). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Microfluidic device for cerebrovascular simulation and high-efficiency blood-brain barrier simulation system comprising same
    Article Snippet: The primary antibodies used in the present disclosure are as follows: anti-CD31 (1:200, Millipore, Billerica, MA, USA), anti-Tuj1 (1:150, Cell Signaling Technology, Danvers, MA, USA), anti-GFAP (1:200, Millipore), anti-O4 (1:500, Millipore), anti-NG2 (1:100, Abcam, Cambridge, UK), anti-M-1 (1:100, Invitrogen) and anti-glucuronoxylomannan (anti-GXM, 1:500, 18B7 mouse monoclonal antibody donated by Dr. Arturo Casadevall in Johns Hopkins University). .. Quantitative Reverse Transcription Polymerase Chain Reaction (qPCR) To compare relative gene expression levels between groups, total RNAs were collected from each sample (n=3, technical replicates=3 per group) using an RNeasy mini kit (Qiagen, Chatsworth, CA, USA), and a complementary deoxyribonucleic acid (cDNA) was synthesized using a cDNA synthesis kit (Takara, Shiga, Japan) as directed by the manufacturer. qPCR analysis was performed on a StepOnePlus Real-Time PCR System (Applied Biosystems) using a TaqMan gene expression assay and a TaqMan Fast Universal PCR Master Mix (Applied Biosystems, Foster City, CA, USA). .. For gene expression analysis in human NSCs, a TaqMan gene expression assay with human-specific primers for the nestin (Hs00707120_s1), Tuj1 (Hs00801390_s1), GFAP (Hs00909238_g1), Olig2 (Hs00300164_s1) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH; Hs02758991_g1) were used.

    Article Title: Remimazolam-induced anesthesia does not affect circadian rhythms significantly in mice.
    Article Snippet: .. 409 Quantitative real-time polymerase chain reaction (q-PCR): Total RNA was 410 extracted from SCN samples using an RNeasy Mini Kit (Qiagen) and reverse-411 transcribed into the complementary deoxyribonucleic acid (cDNA) using a 412 QuantiTect Reverse Transcription Kit (Qiagen). q-PCR was performed using a 413 StepOnePlus Real-Time PCR system (Applied Biosystems, Foster, CA, USA) and a 414 QuantiTect SYBR Green PCR Kit (Qiagen). ..

    Article Title: miR-320a Regulates Placenta Endothelial Function After Fetal Cardiopulmonary Bypass via the ATG7-SIRT1/FOXO1 Pathway.
    Article Snippet: .. Total RNA was reverse-transcribed to complementary deoxyribonucleic acid (cDNA) according to the manufacturer’s protocol (EasyScript First-Strand cDNA Synthesis SuperMix, TransGen Biotech, China). qRT-PCR was performed on a qPCR machine (ABI PRISM® 7500 Sequence Detection System, Applied Biosystems, USA) with SYBR Green qPCR SuperMix (11733–046, Invitrogen, Thermo Scientific, USA). ..

    Article Title: miR-320a Regulates Placenta Endothelial Function After Fetal Cardiopulmonary Bypass via the ATG7-SIRT1/FOXO1 Pathway
    Article Snippet: .. Total RNA was reverse-transcribed to complementary deoxyribonucleic acid (cDNA) according to the manufacturer’s protocol (EasyScript First-Strand cDNA Synthesis SuperMix, TransGen Biotech, China). qRT-PCR was performed on a qPCR machine (ABI PRISM® 7500 Sequence Detection System, Applied Biosystems, USA) with SYBR Green qPCR SuperMix (11733–046, Invitrogen, Thermo Scientific, USA). ..

    Gene Expression:

    Article Title: Microfluidic device for cerebrovascular simulation and high-efficiency blood-brain barrier simulation system comprising same
    Article Snippet: The primary antibodies used in the present disclosure are as follows: anti-CD31 (1:200, Millipore, Billerica, MA, USA), anti-Tuj1 (1:150, Cell Signaling Technology, Danvers, MA, USA), anti-GFAP (1:200, Millipore), anti-O4 (1:500, Millipore), anti-NG2 (1:100, Abcam, Cambridge, UK), anti-M-1 (1:100, Invitrogen) and anti-glucuronoxylomannan (anti-GXM, 1:500, 18B7 mouse monoclonal antibody donated by Dr. Arturo Casadevall in Johns Hopkins University). .. Quantitative Reverse Transcription Polymerase Chain Reaction (qPCR) To compare relative gene expression levels between groups, total RNAs were collected from each sample (n=3, technical replicates=3 per group) using an RNeasy mini kit (Qiagen, Chatsworth, CA, USA), and a complementary deoxyribonucleic acid (cDNA) was synthesized using a cDNA synthesis kit (Takara, Shiga, Japan) as directed by the manufacturer. qPCR analysis was performed on a StepOnePlus Real-Time PCR System (Applied Biosystems) using a TaqMan gene expression assay and a TaqMan Fast Universal PCR Master Mix (Applied Biosystems, Foster City, CA, USA). .. For gene expression analysis in human NSCs, a TaqMan gene expression assay with human-specific primers for the nestin (Hs00707120_s1), Tuj1 (Hs00801390_s1), GFAP (Hs00909238_g1), Olig2 (Hs00300164_s1) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH; Hs02758991_g1) were used.

    Synthesized:

    Article Title: Microfluidic device for cerebrovascular simulation and high-efficiency blood-brain barrier simulation system comprising same
    Article Snippet: The primary antibodies used in the present disclosure are as follows: anti-CD31 (1:200, Millipore, Billerica, MA, USA), anti-Tuj1 (1:150, Cell Signaling Technology, Danvers, MA, USA), anti-GFAP (1:200, Millipore), anti-O4 (1:500, Millipore), anti-NG2 (1:100, Abcam, Cambridge, UK), anti-M-1 (1:100, Invitrogen) and anti-glucuronoxylomannan (anti-GXM, 1:500, 18B7 mouse monoclonal antibody donated by Dr. Arturo Casadevall in Johns Hopkins University). .. Quantitative Reverse Transcription Polymerase Chain Reaction (qPCR) To compare relative gene expression levels between groups, total RNAs were collected from each sample (n=3, technical replicates=3 per group) using an RNeasy mini kit (Qiagen, Chatsworth, CA, USA), and a complementary deoxyribonucleic acid (cDNA) was synthesized using a cDNA synthesis kit (Takara, Shiga, Japan) as directed by the manufacturer. qPCR analysis was performed on a StepOnePlus Real-Time PCR System (Applied Biosystems) using a TaqMan gene expression assay and a TaqMan Fast Universal PCR Master Mix (Applied Biosystems, Foster City, CA, USA). .. For gene expression analysis in human NSCs, a TaqMan gene expression assay with human-specific primers for the nestin (Hs00707120_s1), Tuj1 (Hs00801390_s1), GFAP (Hs00909238_g1), Olig2 (Hs00300164_s1) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH; Hs02758991_g1) were used.

    cDNA Synthesis:

    Article Title: Microfluidic device for cerebrovascular simulation and high-efficiency blood-brain barrier simulation system comprising same
    Article Snippet: The primary antibodies used in the present disclosure are as follows: anti-CD31 (1:200, Millipore, Billerica, MA, USA), anti-Tuj1 (1:150, Cell Signaling Technology, Danvers, MA, USA), anti-GFAP (1:200, Millipore), anti-O4 (1:500, Millipore), anti-NG2 (1:100, Abcam, Cambridge, UK), anti-M-1 (1:100, Invitrogen) and anti-glucuronoxylomannan (anti-GXM, 1:500, 18B7 mouse monoclonal antibody donated by Dr. Arturo Casadevall in Johns Hopkins University). .. Quantitative Reverse Transcription Polymerase Chain Reaction (qPCR) To compare relative gene expression levels between groups, total RNAs were collected from each sample (n=3, technical replicates=3 per group) using an RNeasy mini kit (Qiagen, Chatsworth, CA, USA), and a complementary deoxyribonucleic acid (cDNA) was synthesized using a cDNA synthesis kit (Takara, Shiga, Japan) as directed by the manufacturer. qPCR analysis was performed on a StepOnePlus Real-Time PCR System (Applied Biosystems) using a TaqMan gene expression assay and a TaqMan Fast Universal PCR Master Mix (Applied Biosystems, Foster City, CA, USA). .. For gene expression analysis in human NSCs, a TaqMan gene expression assay with human-specific primers for the nestin (Hs00707120_s1), Tuj1 (Hs00801390_s1), GFAP (Hs00909238_g1), Olig2 (Hs00300164_s1) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH; Hs02758991_g1) were used.

    Article Title: miR-320a Regulates Placenta Endothelial Function After Fetal Cardiopulmonary Bypass via the ATG7-SIRT1/FOXO1 Pathway.
    Article Snippet: .. Total RNA was reverse-transcribed to complementary deoxyribonucleic acid (cDNA) according to the manufacturer’s protocol (EasyScript First-Strand cDNA Synthesis SuperMix, TransGen Biotech, China). qRT-PCR was performed on a qPCR machine (ABI PRISM® 7500 Sequence Detection System, Applied Biosystems, USA) with SYBR Green qPCR SuperMix (11733–046, Invitrogen, Thermo Scientific, USA). ..

    Article Title: miR-320a Regulates Placenta Endothelial Function After Fetal Cardiopulmonary Bypass via the ATG7-SIRT1/FOXO1 Pathway
    Article Snippet: .. Total RNA was reverse-transcribed to complementary deoxyribonucleic acid (cDNA) according to the manufacturer’s protocol (EasyScript First-Strand cDNA Synthesis SuperMix, TransGen Biotech, China). qRT-PCR was performed on a qPCR machine (ABI PRISM® 7500 Sequence Detection System, Applied Biosystems, USA) with SYBR Green qPCR SuperMix (11733–046, Invitrogen, Thermo Scientific, USA). ..

    Article Title: B7 homolog 6 promotes the killing activity of natural killer cells against cervical cancer through the downstream ERK pathway of NKp30
    Article Snippet: Total RNA was extracted from cultured cells using TRIzol ® reagent (cat. no. 213407; Invitrogen; Thermo Fisher Scientific, Inc.). .. Purified RNA was then reverse-transcribed to complementary deoxyribonucleic acid (cDNA) using RevertAid First Strand cDNA Synthesis Kit purchased from Thermo Fisher Scientific, Inc. Next, RT-qPCR was performed using FastStart Universal SYBR Green Master Mix (cat. no. 04913850001; Roche Diagnostics, Basel, Switzerland), following the manufacturer’s instructions. ..

    SYBR Green Assay:

    Article Title: Remimazolam-induced anesthesia does not affect circadian rhythms significantly in mice.
    Article Snippet: .. 409 Quantitative real-time polymerase chain reaction (q-PCR): Total RNA was 410 extracted from SCN samples using an RNeasy Mini Kit (Qiagen) and reverse-411 transcribed into the complementary deoxyribonucleic acid (cDNA) using a 412 QuantiTect Reverse Transcription Kit (Qiagen). q-PCR was performed using a 413 StepOnePlus Real-Time PCR system (Applied Biosystems, Foster, CA, USA) and a 414 QuantiTect SYBR Green PCR Kit (Qiagen). ..

    Article Title: miR-320a Regulates Placenta Endothelial Function After Fetal Cardiopulmonary Bypass via the ATG7-SIRT1/FOXO1 Pathway.
    Article Snippet: .. Total RNA was reverse-transcribed to complementary deoxyribonucleic acid (cDNA) according to the manufacturer’s protocol (EasyScript First-Strand cDNA Synthesis SuperMix, TransGen Biotech, China). qRT-PCR was performed on a qPCR machine (ABI PRISM® 7500 Sequence Detection System, Applied Biosystems, USA) with SYBR Green qPCR SuperMix (11733–046, Invitrogen, Thermo Scientific, USA). ..

    Article Title: miR-320a Regulates Placenta Endothelial Function After Fetal Cardiopulmonary Bypass via the ATG7-SIRT1/FOXO1 Pathway
    Article Snippet: .. Total RNA was reverse-transcribed to complementary deoxyribonucleic acid (cDNA) according to the manufacturer’s protocol (EasyScript First-Strand cDNA Synthesis SuperMix, TransGen Biotech, China). qRT-PCR was performed on a qPCR machine (ABI PRISM® 7500 Sequence Detection System, Applied Biosystems, USA) with SYBR Green qPCR SuperMix (11733–046, Invitrogen, Thermo Scientific, USA). ..

    Article Title: B7 homolog 6 promotes the killing activity of natural killer cells against cervical cancer through the downstream ERK pathway of NKp30
    Article Snippet: Total RNA was extracted from cultured cells using TRIzol ® reagent (cat. no. 213407; Invitrogen; Thermo Fisher Scientific, Inc.). .. Purified RNA was then reverse-transcribed to complementary deoxyribonucleic acid (cDNA) using RevertAid First Strand cDNA Synthesis Kit purchased from Thermo Fisher Scientific, Inc. Next, RT-qPCR was performed using FastStart Universal SYBR Green Master Mix (cat. no. 04913850001; Roche Diagnostics, Basel, Switzerland), following the manufacturer’s instructions. ..

    Quantitative RT-PCR:

    Article Title: miR-320a Regulates Placenta Endothelial Function After Fetal Cardiopulmonary Bypass via the ATG7-SIRT1/FOXO1 Pathway.
    Article Snippet: .. Total RNA was reverse-transcribed to complementary deoxyribonucleic acid (cDNA) according to the manufacturer’s protocol (EasyScript First-Strand cDNA Synthesis SuperMix, TransGen Biotech, China). qRT-PCR was performed on a qPCR machine (ABI PRISM® 7500 Sequence Detection System, Applied Biosystems, USA) with SYBR Green qPCR SuperMix (11733–046, Invitrogen, Thermo Scientific, USA). ..

    Article Title: miR-320a Regulates Placenta Endothelial Function After Fetal Cardiopulmonary Bypass via the ATG7-SIRT1/FOXO1 Pathway
    Article Snippet: .. Total RNA was reverse-transcribed to complementary deoxyribonucleic acid (cDNA) according to the manufacturer’s protocol (EasyScript First-Strand cDNA Synthesis SuperMix, TransGen Biotech, China). qRT-PCR was performed on a qPCR machine (ABI PRISM® 7500 Sequence Detection System, Applied Biosystems, USA) with SYBR Green qPCR SuperMix (11733–046, Invitrogen, Thermo Scientific, USA). ..

    Article Title: B7 homolog 6 promotes the killing activity of natural killer cells against cervical cancer through the downstream ERK pathway of NKp30
    Article Snippet: Total RNA was extracted from cultured cells using TRIzol ® reagent (cat. no. 213407; Invitrogen; Thermo Fisher Scientific, Inc.). .. Purified RNA was then reverse-transcribed to complementary deoxyribonucleic acid (cDNA) using RevertAid First Strand cDNA Synthesis Kit purchased from Thermo Fisher Scientific, Inc. Next, RT-qPCR was performed using FastStart Universal SYBR Green Master Mix (cat. no. 04913850001; Roche Diagnostics, Basel, Switzerland), following the manufacturer’s instructions. ..

    Sequencing:

    Article Title: miR-320a Regulates Placenta Endothelial Function After Fetal Cardiopulmonary Bypass via the ATG7-SIRT1/FOXO1 Pathway.
    Article Snippet: .. Total RNA was reverse-transcribed to complementary deoxyribonucleic acid (cDNA) according to the manufacturer’s protocol (EasyScript First-Strand cDNA Synthesis SuperMix, TransGen Biotech, China). qRT-PCR was performed on a qPCR machine (ABI PRISM® 7500 Sequence Detection System, Applied Biosystems, USA) with SYBR Green qPCR SuperMix (11733–046, Invitrogen, Thermo Scientific, USA). ..

    Article Title: miR-320a Regulates Placenta Endothelial Function After Fetal Cardiopulmonary Bypass via the ATG7-SIRT1/FOXO1 Pathway
    Article Snippet: .. Total RNA was reverse-transcribed to complementary deoxyribonucleic acid (cDNA) according to the manufacturer’s protocol (EasyScript First-Strand cDNA Synthesis SuperMix, TransGen Biotech, China). qRT-PCR was performed on a qPCR machine (ABI PRISM® 7500 Sequence Detection System, Applied Biosystems, USA) with SYBR Green qPCR SuperMix (11733–046, Invitrogen, Thermo Scientific, USA). ..

    Purification:

    Article Title: B7 homolog 6 promotes the killing activity of natural killer cells against cervical cancer through the downstream ERK pathway of NKp30
    Article Snippet: Total RNA was extracted from cultured cells using TRIzol ® reagent (cat. no. 213407; Invitrogen; Thermo Fisher Scientific, Inc.). .. Purified RNA was then reverse-transcribed to complementary deoxyribonucleic acid (cDNA) using RevertAid First Strand cDNA Synthesis Kit purchased from Thermo Fisher Scientific, Inc. Next, RT-qPCR was performed using FastStart Universal SYBR Green Master Mix (cat. no. 04913850001; Roche Diagnostics, Basel, Switzerland), following the manufacturer’s instructions. ..

    Produced:

    Article Title: Lunar life drives jawbone formation
    Article Snippet: We used a ClariomTM S Assay Mouse (Thermo Fisher Scientific, # 902,930) for this assay. .. First, we produced fragmented and labeled complementary deoxyribonucleic acid (cDNA) from 50 ng of total RNA with a GeneChipTM 3′ IVT Pico Kit (Thermo Fisher Scientific, # 902,789) and prepared 100 μL of hybridization mixture (73 μL of hybridization master mix + 5.0 μg of fragmented and labeled cDNA). .. The arrays were incubated for 16 h (60 rpm) at 45 °C in a GeneChipTM Hybridization Oven 645 (Thermo Fisher Scientific) and washed with a GeneChipTM Fluidics Station 450 (Thermo Fisher Scientific) according to the manufacturer's protocol.

    Labeling:

    Article Title: Lunar life drives jawbone formation
    Article Snippet: We used a ClariomTM S Assay Mouse (Thermo Fisher Scientific, # 902,930) for this assay. .. First, we produced fragmented and labeled complementary deoxyribonucleic acid (cDNA) from 50 ng of total RNA with a GeneChipTM 3′ IVT Pico Kit (Thermo Fisher Scientific, # 902,789) and prepared 100 μL of hybridization mixture (73 μL of hybridization master mix + 5.0 μg of fragmented and labeled cDNA). .. The arrays were incubated for 16 h (60 rpm) at 45 °C in a GeneChipTM Hybridization Oven 645 (Thermo Fisher Scientific) and washed with a GeneChipTM Fluidics Station 450 (Thermo Fisher Scientific) according to the manufacturer's protocol.

    Hybridization:

    Article Title: Lunar life drives jawbone formation
    Article Snippet: We used a ClariomTM S Assay Mouse (Thermo Fisher Scientific, # 902,930) for this assay. .. First, we produced fragmented and labeled complementary deoxyribonucleic acid (cDNA) from 50 ng of total RNA with a GeneChipTM 3′ IVT Pico Kit (Thermo Fisher Scientific, # 902,789) and prepared 100 μL of hybridization mixture (73 μL of hybridization master mix + 5.0 μg of fragmented and labeled cDNA). .. The arrays were incubated for 16 h (60 rpm) at 45 °C in a GeneChipTM Hybridization Oven 645 (Thermo Fisher Scientific) and washed with a GeneChipTM Fluidics Station 450 (Thermo Fisher Scientific) according to the manufacturer's protocol.



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    Thermo Fisher complementary dna preparation
    Nucleolar-targeting compounds inhibit nucleolar activity and induce selective <t>DNA</t> damage. ( A ) Representative images of EU incorporation (green) in THLE-3, PLC/PRF/5, and SNU-449 cells 24 h after treatment with four anti-cancer drugs targeting the nucleolus: CX-5461, BMH-21, Oxaliplatin, and JP-1302, or targeted therapy Sorafenib. Cells were treated with DMSO as control and were stained with DAPI (blue) and Pol II (yellow). ( B ) Mean nucleolar EU intensity per nucleus as a readout for rDNA <t>transcription</t> in THLE-3, PLC/PRF/5, and SNU-449 cells 24 h after treatment with four anti-cancer drugs targeting the nucleolus: CX-5461, BMH-21, Oxaliplatin, and JP-1302, or targeted therapy Sorafenib. Cells were treated with DMSO as control. The graph depicts one representative biological replicate ( n = 3), with data points representing individual cells, and the median indicated by the red line. A Kruskal–Wallis test was applied to assess differences between groups for the replicates combined, and statistical significance is indicated by numerical P -values. ( C ) Representative images of nuclear 53BP1 and γH2AX foci accumulation in THLE-3, PLC/PRF/5, and SNU-449 cells 24 h after treatment with four anti-cancer drugs targeting the nucleolus: CX-5461, BMH-21, Oxaliplatin, and JP-1302, or targeted therapy Sorafenib. Cells were treated with DMSO as control, and stained with DAPI (blue), 53BP1 (yellow), and γH2AX (red). ( D ) Fold change of nuclear 53BP1 foci per cell relative to DMSO. Individual points in the graph represent biological replicates ( n = 3). The graph depicts the mean ± SD and statistical significance was assessed by one sample t test and indicated by numerical P -values. ( E ) Fold change of nuclear γH2AX foci per cell relative to DMSO otherwise as in panel (D).
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    Nucleolar-targeting compounds inhibit nucleolar activity and induce selective <t>DNA</t> damage. ( A ) Representative images of EU incorporation (green) in THLE-3, PLC/PRF/5, and SNU-449 cells 24 h after treatment with four anti-cancer drugs targeting the nucleolus: CX-5461, BMH-21, Oxaliplatin, and JP-1302, or targeted therapy Sorafenib. Cells were treated with DMSO as control and were stained with DAPI (blue) and Pol II (yellow). ( B ) Mean nucleolar EU intensity per nucleus as a readout for rDNA <t>transcription</t> in THLE-3, PLC/PRF/5, and SNU-449 cells 24 h after treatment with four anti-cancer drugs targeting the nucleolus: CX-5461, BMH-21, Oxaliplatin, and JP-1302, or targeted therapy Sorafenib. Cells were treated with DMSO as control. The graph depicts one representative biological replicate ( n = 3), with data points representing individual cells, and the median indicated by the red line. A Kruskal–Wallis test was applied to assess differences between groups for the replicates combined, and statistical significance is indicated by numerical P -values. ( C ) Representative images of nuclear 53BP1 and γH2AX foci accumulation in THLE-3, PLC/PRF/5, and SNU-449 cells 24 h after treatment with four anti-cancer drugs targeting the nucleolus: CX-5461, BMH-21, Oxaliplatin, and JP-1302, or targeted therapy Sorafenib. Cells were treated with DMSO as control, and stained with DAPI (blue), 53BP1 (yellow), and γH2AX (red). ( D ) Fold change of nuclear 53BP1 foci per cell relative to DMSO. Individual points in the graph represent biological replicates ( n = 3). The graph depicts the mean ± SD and statistical significance was assessed by one sample t test and indicated by numerical P -values. ( E ) Fold change of nuclear γH2AX foci per cell relative to DMSO otherwise as in panel (D).
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    Nucleolar-targeting compounds inhibit nucleolar activity and induce selective <t>DNA</t> damage. ( A ) Representative images of EU incorporation (green) in THLE-3, PLC/PRF/5, and SNU-449 cells 24 h after treatment with four anti-cancer drugs targeting the nucleolus: CX-5461, BMH-21, Oxaliplatin, and JP-1302, or targeted therapy Sorafenib. Cells were treated with DMSO as control and were stained with DAPI (blue) and Pol II (yellow). ( B ) Mean nucleolar EU intensity per nucleus as a readout for rDNA <t>transcription</t> in THLE-3, PLC/PRF/5, and SNU-449 cells 24 h after treatment with four anti-cancer drugs targeting the nucleolus: CX-5461, BMH-21, Oxaliplatin, and JP-1302, or targeted therapy Sorafenib. Cells were treated with DMSO as control. The graph depicts one representative biological replicate ( n = 3), with data points representing individual cells, and the median indicated by the red line. A Kruskal–Wallis test was applied to assess differences between groups for the replicates combined, and statistical significance is indicated by numerical P -values. ( C ) Representative images of nuclear 53BP1 and γH2AX foci accumulation in THLE-3, PLC/PRF/5, and SNU-449 cells 24 h after treatment with four anti-cancer drugs targeting the nucleolus: CX-5461, BMH-21, Oxaliplatin, and JP-1302, or targeted therapy Sorafenib. Cells were treated with DMSO as control, and stained with DAPI (blue), 53BP1 (yellow), and γH2AX (red). ( D ) Fold change of nuclear 53BP1 foci per cell relative to DMSO. Individual points in the graph represent biological replicates ( n = 3). The graph depicts the mean ± SD and statistical significance was assessed by one sample t test and indicated by numerical P -values. ( E ) Fold change of nuclear γH2AX foci per cell relative to DMSO otherwise as in panel (D).
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    Nucleolar-targeting compounds inhibit nucleolar activity and induce selective <t>DNA</t> damage. ( A ) Representative images of EU incorporation (green) in THLE-3, PLC/PRF/5, and SNU-449 cells 24 h after treatment with four anti-cancer drugs targeting the nucleolus: CX-5461, BMH-21, Oxaliplatin, and JP-1302, or targeted therapy Sorafenib. Cells were treated with DMSO as control and were stained with DAPI (blue) and Pol II (yellow). ( B ) Mean nucleolar EU intensity per nucleus as a readout for rDNA <t>transcription</t> in THLE-3, PLC/PRF/5, and SNU-449 cells 24 h after treatment with four anti-cancer drugs targeting the nucleolus: CX-5461, BMH-21, Oxaliplatin, and JP-1302, or targeted therapy Sorafenib. Cells were treated with DMSO as control. The graph depicts one representative biological replicate ( n = 3), with data points representing individual cells, and the median indicated by the red line. A Kruskal–Wallis test was applied to assess differences between groups for the replicates combined, and statistical significance is indicated by numerical P -values. ( C ) Representative images of nuclear 53BP1 and γH2AX foci accumulation in THLE-3, PLC/PRF/5, and SNU-449 cells 24 h after treatment with four anti-cancer drugs targeting the nucleolus: CX-5461, BMH-21, Oxaliplatin, and JP-1302, or targeted therapy Sorafenib. Cells were treated with DMSO as control, and stained with DAPI (blue), 53BP1 (yellow), and γH2AX (red). ( D ) Fold change of nuclear 53BP1 foci per cell relative to DMSO. Individual points in the graph represent biological replicates ( n = 3). The graph depicts the mean ± SD and statistical significance was assessed by one sample t test and indicated by numerical P -values. ( E ) Fold change of nuclear γH2AX foci per cell relative to DMSO otherwise as in panel (D).
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    Image Search Results


    Nucleolar-targeting compounds inhibit nucleolar activity and induce selective DNA damage. ( A ) Representative images of EU incorporation (green) in THLE-3, PLC/PRF/5, and SNU-449 cells 24 h after treatment with four anti-cancer drugs targeting the nucleolus: CX-5461, BMH-21, Oxaliplatin, and JP-1302, or targeted therapy Sorafenib. Cells were treated with DMSO as control and were stained with DAPI (blue) and Pol II (yellow). ( B ) Mean nucleolar EU intensity per nucleus as a readout for rDNA transcription in THLE-3, PLC/PRF/5, and SNU-449 cells 24 h after treatment with four anti-cancer drugs targeting the nucleolus: CX-5461, BMH-21, Oxaliplatin, and JP-1302, or targeted therapy Sorafenib. Cells were treated with DMSO as control. The graph depicts one representative biological replicate ( n = 3), with data points representing individual cells, and the median indicated by the red line. A Kruskal–Wallis test was applied to assess differences between groups for the replicates combined, and statistical significance is indicated by numerical P -values. ( C ) Representative images of nuclear 53BP1 and γH2AX foci accumulation in THLE-3, PLC/PRF/5, and SNU-449 cells 24 h after treatment with four anti-cancer drugs targeting the nucleolus: CX-5461, BMH-21, Oxaliplatin, and JP-1302, or targeted therapy Sorafenib. Cells were treated with DMSO as control, and stained with DAPI (blue), 53BP1 (yellow), and γH2AX (red). ( D ) Fold change of nuclear 53BP1 foci per cell relative to DMSO. Individual points in the graph represent biological replicates ( n = 3). The graph depicts the mean ± SD and statistical significance was assessed by one sample t test and indicated by numerical P -values. ( E ) Fold change of nuclear γH2AX foci per cell relative to DMSO otherwise as in panel (D).

    Journal: NAR Cancer

    Article Title: Ribosome biogenesis is increased in hepatocellular carcinoma and represents a potential therapeutic target

    doi: 10.1093/narcan/zcaf058

    Figure Lengend Snippet: Nucleolar-targeting compounds inhibit nucleolar activity and induce selective DNA damage. ( A ) Representative images of EU incorporation (green) in THLE-3, PLC/PRF/5, and SNU-449 cells 24 h after treatment with four anti-cancer drugs targeting the nucleolus: CX-5461, BMH-21, Oxaliplatin, and JP-1302, or targeted therapy Sorafenib. Cells were treated with DMSO as control and were stained with DAPI (blue) and Pol II (yellow). ( B ) Mean nucleolar EU intensity per nucleus as a readout for rDNA transcription in THLE-3, PLC/PRF/5, and SNU-449 cells 24 h after treatment with four anti-cancer drugs targeting the nucleolus: CX-5461, BMH-21, Oxaliplatin, and JP-1302, or targeted therapy Sorafenib. Cells were treated with DMSO as control. The graph depicts one representative biological replicate ( n = 3), with data points representing individual cells, and the median indicated by the red line. A Kruskal–Wallis test was applied to assess differences between groups for the replicates combined, and statistical significance is indicated by numerical P -values. ( C ) Representative images of nuclear 53BP1 and γH2AX foci accumulation in THLE-3, PLC/PRF/5, and SNU-449 cells 24 h after treatment with four anti-cancer drugs targeting the nucleolus: CX-5461, BMH-21, Oxaliplatin, and JP-1302, or targeted therapy Sorafenib. Cells were treated with DMSO as control, and stained with DAPI (blue), 53BP1 (yellow), and γH2AX (red). ( D ) Fold change of nuclear 53BP1 foci per cell relative to DMSO. Individual points in the graph represent biological replicates ( n = 3). The graph depicts the mean ± SD and statistical significance was assessed by one sample t test and indicated by numerical P -values. ( E ) Fold change of nuclear γH2AX foci per cell relative to DMSO otherwise as in panel (D).

    Article Snippet: Reverse transcription polymerase chain reaction (RT-PCR) was performed using the complementary DNA (cDNA) transcription kit with random primers (Thermo Fisher #4368814). cDNA was subjected to quantitative PCR (qPCR) using the Applied Biosystems 7500 Fast Real-Time PCR system with Fast SYBR TM Green Master Mix (Applied Biosystems #4385612) for detection.

    Techniques: Activity Assay, Control, Staining